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Recombinant Pup--protein ligase (pafA)

Recombinant Pup--protein ligase (pafA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP509881XBKYeast1mgQuote
EP509881XBKE. coli1mgQuote
BP509881XBKBaculovirus200ugQuote
MP509881XBKMammalian Cell200ugQuote

Protein Information

SpeciesXylanimonas cellulosilytica (strain DSM 15894 / CECT 5975 / LMG 20990 / XIL07)
UniProt IDD1BS28
Gene NamepafA; Locus:Xcel_1490
Protein NamePup--protein ligase
Region Expressed1-457
Expression Tag6xHis
Purity>90%
AA SequenceMDRRIFGIETEYGVTCAAPDGRGLSADEVARYLFRKVVAWGRSSNVFLRNGSRLYLDVGS HPEYATAECDDVRQLVTYDKGGERILEGLVADAQQRLEHEGLPGTVHLFKNNTDSAGNSY GCHENYLVRRQGDFARLSDILVPFLITRQVLVGAGKVLATPRGAVYCLSQRADHIWEAVS SATTRSRPIINTRDEPHADAEYFRRLHVIVGDSSMAEPTTMLKVGATDLVLRLVEAGVIM RDLSLENPIRAIREISHDRTGTHAVQLADGRTMTAVDIQGEYFRRVREHVDAHGINDADP SPTTKAVLDLWERGLHALESGDLSSVDRELDWVIKLRLIERYQAKHGLELDDPRIARLDL AYHDISRTEGLYNLLAARGMVERVTTDLEILESTAVPPPTTRAKLRGDFVRAAQDARRDY TVDWVHLKLNDQAQRTVLCKDPFRSVDERVDRLIESM
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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