Request QuoteCatalog Number: xP500120DPYSize: 0.2-1mg

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Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)

Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP500120DPYYeast1mgQuote
EP500120DPYE. coli1mgQuote
BP500120DPYBaculovirus200ugQuote
MP500120DPYMammalian Cell200ugQuote

Protein Information

SpeciesAzotobacter vinelandii (strain DJ / ATCC BAA-1303)
UniProt IDC1DSS0
Gene NameispF; Locus:Avin_38730
Protein Name2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Region Expressed1-157
Expression Tag6xHis
Purity>90%
AA SequenceMRIGHGYDVHRFAEGDFVTLGGVRIPHRFGLLAHSDGDVLLHALCDALLGAAALGDIGRH FPDTDPRFKGVDSRVLLRHVVALVREKGWRVGNVDATIVAQAPKMAPHIERMRLSIAEDL QVGVEQVNVKATTTEKLGFTGREEGIAVHAVALLLAL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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