$90.00Catalog Number: PYT370-5ugSize: 5ug

IL 2 Mouse

Interleukin-2 Mouse Recombinant

Interleukin-2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 148 amino acids and having a molecular mass of 17231 Dalton.
The IL-2 is purified by proprietary chromatographic techniques.

Accession

P04351

Amino acid sequence

PTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDLQELLSR MENYRNLKLP RMLTFKFYLP KQATELKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ FDDESATVVD FLRRWIAFCQ SIISTSPQ.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is 0.18 ng/ml corresponding to a Specific Activity of 55MIU/mg.

Formulation

The protein (1mg/ml) was lyophilized extensive dialysis against 10mM sodium citrate buffer to a pH=4.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content

Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.482 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.

Purity

Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Mouse IL-2 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IL2 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid multiple freeze-thaw cycles.

Synonyms

Interleukin-2, T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.

Quantity

$90.00

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