$90.00Catalog Number: PCM280-2ugSize: 2ug

MCP 3 Rat

Monocyte Chemotactic Protein-3 Rat Recombinant (CCL7)

MCP-3 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of 8.5kDa. The MCP-3 is purified by proprietary chromatographic techniques.

Accession

Q9QXY8

Amino acid sequence

QPDGTNSSTC CYVKKQKIPK RNLKSYRKIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTS TPKP.

Biological Activity

Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 50.0-300.0 ng/ml.

Formulation

MCP-3 protein was lyophilized from a 0.2um filtered concentrated solution in 20mM PB, 300mM NaCl, pH 7.4.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized MCP-3 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized MCP-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution MCP3 should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

C-C motif chemokine 7, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, MCP-3, Small-inducible cytokine A7, Ccl7, Mcp3, Scya7.

Quantity

$90.00

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