Request QuoteCatalog Number: xP492331DJRSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP492331DJRYeast1mgQuote
EP492331DJRE. coli1mgQuote
BP492331DJRBaculovirus200ugQuote
MP492331DJRMammalian Cell200ugQuote

Protein Information

SpeciesDesulfovibrio vulgaris (strain Miyazaki F / DSM 19637)
UniProt IDB8DM91
Gene NameleuA; Locus:DvMF_1791
Protein Name2-isopropylmalate synthase
Region Expressed1-514
Expression Tag6xHis
Purity>90%
AA SequenceMTIESGRIRIFDTTLRDGEQSPGATMNLQEKIRLARQLETLGVDIMEAGFPASSQGDFEA VQAIARAVKGVEVAGLCRAMPADIDRAWEAVKVAENPRIHTFLATSPVHMQYKLRKEPDQ VVEMAVAAVRHAAKYTSNVEFSAEDASRSNPDFLVRVFEAVINAGATTINVPDTVGYAQP EEFGRLIRYVIENTPNSHKAVFSVHCHNDLGMGVANTLAALKAGARQAEVTISGIGERAG NASLEEIVMALHTRRDFYQLDCGVVTEQLFPTCRLLSMIIGQPIPPNKAIVGANAFAHES GIHQDGMLKNRETYEIMTPESIGKTKTDLVIGKHSGRNAVKNKLDELGYRLEEAQLVTVF EAVKKLADKKKQIYDEDIEALVLEEVYRLPDLYRLVNLSVQCSDTGMPPTAAVVMDVMGE VKRAAGFGVGPIDAVFNVIGEIVGRAPVLERYSVTAITGGTDAQGEVTVRLRQNGSSAVG RGSDPDIILASARAYVNALNRLAKKEEEQEKEGI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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