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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP502222RKXYeast1mgQuote
EP502222RKXE. coli1mgQuote
BP502222RKXBaculovirus200ugQuote
MP502222RKXMammalian Cell200ugQuote

Protein Information

SpeciesRhizobium sp. (strain NGR234)
UniProt IDC3M9V0
Gene NameleuC; Locus:NGR_c31400
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-469
Expression Tag6xHis
Purity>90%
AA SequenceMSAPRTLYDKIWDDHLVNQQDDGTCLLYIDRHLVHEVTSPQAFEGLRMAGRKVRAPEKTL AVVDHNVPTSPDRHLGIKNEESRIQVEALARNAADFGVEYYSENDKRQGIVHIVGPEQGF TLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGQLPPG VTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTICNMTIEGGARAGLIAPDE TTFDYIKDKPRAPKGKAWDMALDYWKTLHTDEGAHYDRIVVLDAADLPPIVSWGSSPEDV ISVQGAVPNPDDIQEETKRASKWRALDYMGLKPGTKITDIAVDRVFIGSCTNGRIEDLRA VAKVVEGRKVASTVSAMIVPGSGLVKEQAEAEGLDKIFKEAGFDWREPGCSMCLAMNDDR LKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAVAGHFVDIREWK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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