$90.00Catalog Number: PYT048-1ugSize: 1ug

IL 27 Human

Interleukin-27 Human Recombinant

Interleukin-27 Human Recombinant produced in HEK 293 is a glycosylated non-covalently linked heterodimer, consisting of one p28 subunit (215 amino acids) and one EBI3 subunit (209 amino acids), with a total a molecular weight of 47.8kDa.
Human IL27 is purified by proprietary chromatographic techniques.

Accession

Q8NEV9

Amino acid sequence

EBI3: RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRRRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGKP28: FPRPPGRPQL SLQELRREFT VSLHLARKLL SEVRGQAHRF AESHLPGVNL YLLPLGEQLP DVSLTFQAWR RLSDPERLCF ISTTLQPFHA LLGGLGTQGR WTNMERMQLW AMRLDLRDLQ RHLRFQVLAA GFNLPEEEEE EEEEEEEERK GLLPGALGSA LQGPAQVSWP QLLSTYRLLH SLELVLSRAV RELLLLSKAG HSVWPLGFPT LSPQP

Biological Activity

The activity is determined by its ability to inhibit IL-2 production from activated mouse spleenocytes and is typically 60-90ng/mL.

Formulation

Lyophilized from 10mM Na2PO4 + 50mM NaCl, pH 7.5.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(c) UV spectroscopy at 280 nm.

Solubility

It is recommended to reconstitute the lyophilized Interleukin-27 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

HEK 293 cells

Stability

Lyophilized Interleukin-27 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IL27 should be stored at 4oC between 2-7 days and for future use below -18oC.
Please avoid multiple freeze-thaw cycles.

Synonyms

Interleukin-30, IL-30, IL27/p28, p28, Interleukin-27, Interleukin-27/p28, IL27, Interleukin-27 subunit alpha, IL27 subunit alpha, IL27-A, Il27, Il27a, IL27p28.

Quantity

$90.00

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