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Recombinant E3 ubiquitin-protein ligase RFWD3 (RFWD3)

Recombinant E3 ubiquitin-protein ligase RFWD3 (RFWD3) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP019609AYXYeast1mgQuote
EP019609AYXE. coli1mgQuote
BP019609AYXBaculovirus200ugQuote
MP019609AYXMammalian Cell200ugQuote

Protein Information

SpeciesAiluropoda melanoleuca (Giant panda)
UniProt IDD2HWM5
Gene NameRFWD3; aka: RNF201; ORFs:PANDA_016934
Protein NameE3 ubiquitin-protein ligase RFWD3
Region Expressed1-773
Expression Tag6xHis
Purity>90%
AA SequenceMAQEAMEYNVDEQLEHRVAEQPVPAEVVSTQGGPPPLQPLPTEVVSSQGAPPLLQPAPAE GTSSQVGPHLLQPAAQLSVDLTEEVELLGEDRVENINPGASEEHRQPSRVNRPIPVSSLD SMNSFISGLQRLHGMLEFLRPPSDHNVGPVRSRRRRGSASRRSRTVGSQRTDSARSRAPL DAYFQVSRTQPHLPSMSQDSETRNPVSEDLQVSSSSSSDSESSAEYEEVVVQAEDTRAVV SEEQGGTAAEQEVTCVGGGETLPKQSPQKTNPLLPSVSKDDEEGDTCTICFEHWTNAGDH RLSALRCGHLFGYKCISKWLKGQARKCPQCNKKAKHSDIVVLYARTLRALDTSEHERMKS SLLKEQMLRKQAELESAQCRLQLQVLTDECSKLHSRVQDLQKLTVQHRDQISQSPSGSQA RSLNCLPSSQNQRKYHFQKTFTVSPTGNCRIMTYCDALSCLVVSQPSPQASFLPGFGVKM LSTANMKSSQYVPMHGKQIRGLAFSSRSKGLLLSASLDSTVKLTSLETNTVVQTYNAGRP VWSCCWCLDESNHIYAGLVNGSILVYDLRNTSSHIQELVPQKARCPLVSLSYIPRAASAA FPYGGVLAGTLENASFWELKMGFSHWPHVLPMEPGGCVDFQTESSTRHCLVTYRPDKNHN TLRSVLMEMSYKLNDAGEPVCSCRPVQTFLGGPTCKLLTKSAIFQNPENDGSILVCTGDE ASNSALLWDAGSGSLLQELQADQPVLDICPFEANHSSCLATLTEKMVHIYRWE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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