Request QuoteCatalog Number: xP388440RIJSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP388440RIJYeast1mgQuote
EP388440RIJE. coli1mgQuote
BP388440RIJBaculovirus200ugQuote
MP388440RIJMammalian Cell200ugQuote

Protein Information

SpeciesRhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9)
UniProt IDA3PPW5
Gene NamemiaB; Locus:Rsph17029_3285
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-436
Expression Tag6xHis
Purity>90%
AA SequenceMSEARKLFIKTYGCQMNVYDSERMAEALGAKGYVLTEVAEEADMVLLNTCHIREKAAEKV YSDLGRLRPLKTAKPDLKIGVAGCVAQAEGEEILKRMPLVDLVVGPQSYHRLPDMLDRTE GGARVVDTDFPEEDKFDHLPERKATRGPAAFLTVQEGCDKFCAFCVVPYTRGAEVSRPFA RLMAEARGLVERGVREITLLGQNVNAWSSDGRGLGGLIRELARIDGLERLRYTTSHPNDM ADDLIEAHGAEPKLMPYLHLPVQSGSDRILKAMNRKHTAEHYLRLIERIRAARPDILLTS DFIVGFPGETEADFEATLDLIRAVGFGSAFSFKYSARPGTPAAEKPELPGEVCDARLQRL QALVTEQQRAAQMAMVGREVGVLYEKAGRLPGQMVGKSDHLHAVHVEDKAGRVGDLVRVR ITASAPNSLAGERLGA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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