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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP417343CWUYeast1mgQuote
EP417343CWUE. coli1mgQuote
BP417343CWUBaculovirus200ugQuote
MP417343CWUMammalian Cell200ugQuote

Protein Information

SpeciesClostridium botulinum (strain ATCC 19397 / Type A)
UniProt IDA7FUL1
Gene NamemiaB; Locus:CLB_1736
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-450
Expression Tag6xHis
Purity>90%
AA SequenceMNEILNTKDINVIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCC VRENAELKVYGNLGILKGLKSKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNL HNFPNYLNEVKKKDTSVLKIQEKENSIIENMPIDRKNSMKAFVTIMYGCNNFCTYCIVPY VRGRERSRTPENIEAEIKKLISEGYKEITLLGQNVNSYGKDLEPNVTFAELLKRVNNIEG LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVV SKIKKLIPNVALSTDIIVGFPGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFE DQVPEDVKHKRFNRLVEVLNEISAKKNKAYEGKIEEVLVEGTSKNDENKLMGRTRTGKLV NFIGDKDSIGELVNVKIIKANSFSLTGEEI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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