WNT Inhibitory Factor 1 Human Recombinant
WIF1 produced in Hi-5 cells is a single, glycosylated polypeptide chain containing 369 amino acids (29-379 a.a.) and having a molecular mass of 40.5 kDa, (48kDa on SDS-PAGE).
WIF1 is fused to 16 amino acid His Tag at C-Terminus and purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
ADLGPPQEES LYLWIDAHQA RVLIGFEEDI LIVSEGKMAP FTHDFRKAQQ RMPAIPVNIH SMNFTWQAAG QAEYFYEFLS LRSLDKGIMA DPTVNVPLLG TVPHKASVVQ VGFPCLGKQD GVAAFEVDVI VMNSEGNTIL KTPQNAIFFK TCQQAECPGG CRNGGFCNER RICECPDGFH GPHCEKALCT PRCMNGGLCV TPGFCICPPG FYGVNCDKAN CSTTCFNGGT CFYPGKCICP PGLEGEQCEI SKCPQPCRNG GKCIGKSKCK CSKGYQGDLC SKPVCEPGCG AHGTCHEPNK CQCQEGWHGR HCNKRYEASL IHALRPAGAQ LRQHTPSLKK AEERRDPPES NYIWSGRLVP RGSHHHHHH.
Formulation
The WIF1 protein solution contains 1X PBS pH 7.4, 10% glycerol and 1mM PMSF.
Physical Appearance
Sterile filtered colorless solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Source
Stability
Store at 4oC if entire vial will be used within 2-4 weeks.
Store, frozen at -20oC for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Synonyms
WIF1, WIF-1, Wnt inhibitory factor 1.
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