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Recombinant Vitamin B12 transporter BtuB (btuB)

Recombinant Vitamin B12 transporter BtuB (btuB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP424092EJDYeast1mgQuote
EP424092EJDE. coli1mgQuote
BP424092EJDBaculovirus200ugQuote
MP424092EJDMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli O139:H28 (strain E24377A / ETEC)
UniProt IDA7ZUI6
Gene NamebtuB; Locus:EcE24377A_4507
Protein NameVitamin B12 transporter BtuB
Region Expressed21-614
Expression Tag6xHis
Purity>90%
AA SequenceQDTSPDTLVVTANRFEQPRSTVLAPTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGS GQLSSIFIRGTNASHVLVLIDGVRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGS DAIGGVVNIITTRDEPGTEISAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDV VAYGNTGTQAQTDNDGFLSKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLL DTRKLYSQSWDAGLRYNGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQW ANNVIVGHGSIGAGVDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARND DNSQFGRHGTWQTSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEG AFEGLTAGVNWRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVS YDYVDARNAITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVK MGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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