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Recombinant Vitamin B12 transporter BtuB (btuB)

Recombinant Vitamin B12 transporter BtuB (btuB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP396387EIZYeast1mgQuote
EP396387EIZE. coli1mgQuote
BP396387EIZBaculovirus200ugQuote
MP396387EIZMammalian Cell200ugQuote

Protein Information

SpeciesEnterobacter sp. (strain 638)
UniProt IDA4WG45
Gene NamebtuB; Locus:Ent638_4020
Protein NameVitamin B12 transporter BtuB
Region Expressed21-615
Expression Tag6xHis
Purity>90%
AA SequenceQDSADSLVVTANRFEQPANTVLAPTSVVTREDIERWQSTTVLDVMRRLPGVDTAQNGGMG QASSLFVRGTNSSHVLILVDGIRLNQAGVTGSSDLSQFPISLVQRIEYIRGPRSAVYGSD AIGGVVNIITTRAKDGTTLNAGVGSHGYQNYGGSTQQTLGDNTRVTLAGDYTYTKGFDVV ADGNNGGLAQTDRDGYMNKTIYGALEHAFSDQWSGFVRGFGYSNRTAYDAYYSSFTPDVL VDTRQLYSQTWDTGLRFNDGIFHSQLLSSYSHSKDYNYDPHLGRYDSTATLDEIKQYNVQ WTNSVDVGHGNVGAGVDWQKQSTEPGTNYVTNDTNLRNTGVYLTALQKFGDFTLEGAARS DDNSQFGRHGTWQSSAAWEFIEGYRFIASYGTAYKAPNLGQLYGFYGNDHLNPEESKQWE GAFEGLTAGVSWRVSAYRNDVDNLIDFDSNLQQYYNVGKARIKGVEATASFDTGPLTHTV GYDYVDARNAATNELLDRRAKQQVKYQLDTQVYDFDWSLTYHYLGTRYDTDFGSYPYQKV KMGGVSLWDLAVSYPVTSHLTVRGKIANLFDKDYETVYGYETAGREYTLSGSYTF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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