Request QuoteCatalog Number: xP511533ENUSize: 0.2-1mg

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Recombinant Vitamin B12 import ATP-binding protein BtuD (btuD)

Recombinant Vitamin B12 import ATP-binding protein BtuD (btuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP511533ENUYeast1mgQuote
EP511533ENUE. coli1mgQuote
BP511533ENUBaculovirus200ugQuote
MP511533ENUMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli (strain K12 / MC4100 / BW2952)
UniProt IDC4ZYH1
Gene NamebtuD; Locus:BWG_1523
Protein NameVitamin B12 import ATP-binding protein BtuD
Region Expressed1-249
Expression Tag6xHis
Purity>90%
AA SequenceMSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLARMAGMTSGKGSIQFAG QPLEAWSATKLALHRAYLSQQQTPPFATPVWHYLTLHQHDKTRTELLNDVAGALALDDKL GRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEPMNSLDVAQQSALDKILSALC QQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRREEVLTPPNLAQAYGMNFRRLDIE GHRMLISTI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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