Request QuoteCatalog Number: xP474129ENWSize: 0.2-1mg

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Recombinant Vitamin B12 import ATP-binding protein BtuD (btuD)

Recombinant Vitamin B12 import ATP-binding protein BtuD (btuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP474129ENWYeast1mgQuote
EP474129ENWE. coli1mgQuote
BP474129ENWBaculovirus200ugQuote
MP474129ENWMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli (strain SE11)
UniProt IDB6I8R4
Gene NamebtuD; Locus:ECSE_1834
Protein Name
Region Expressed1-249
Expression Tag6xHis
Purity>90%
AA SequenceMSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLARMAGMTSGKGSIQFAG QPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKTRTELLNDVAGALALDDKL GRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEPMNSLDVAQQSALDKILSALC QQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRREEVLTPPNLAQAYGMNFRRLDIE GHRMLISTI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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