Request QuoteCatalog Number: xP503983FGUSize: 0.2-1mg

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Recombinant Vitamin D (3) 25-hydroxylase

Recombinant Vitamin D (3) 25-hydroxylase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP503983FGUYeast1mgQuote
EP503983FGUE. coli1mgQuote
BP503983FGUBaculovirus200ugQuote
MP503983FGUMammalian Cell200ugQuote

Protein Information

SpeciesPseudonocardia autotrophica (Amycolata autotrophica) (Nocardia autotrophica)
UniProt IDC4B644
Gene Namevdh
Protein NameVitamin D (3) 25-hydroxylase
Region Expressed2-403
Expression Tag6xHis
Purity>90%
AA SequenceALTTTGTEQHDLFSGTFWQNPHPAYAALRAEDPVRKLALPDGPVWLLTRYADVREAFVDP RLSKDWRHTLPEDQRADMPATPTPMMILMDPPDHTRLRKLVGRSFTVRRMNELEPRITEI ADGLLAGLPTDGPVDLMREYAFQIPVQVICELLGVPAEDRDDFSAWSSVLVDDSPADDKN AAMGKLHGYLSDLLERKRTEPDDALLSSLLAVSDEDGDRLSQEELVAMAMLLLIAGHETT VNLIGNGVLALLTHPDQRKLLAEDPSLISSAVEEFLRFDSPVSQAPIRFTAEDVTYSGVT IPAGEMVMLGLAAANRDADWMPEPDRLDITRDASGGVFFGHGIHFCLGAQLARLEGRVAI GRLFADRPELALAVGLDELVYRESTLVRGLSRMPVTMGPRSA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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