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Recombinant Vitamin D (3) 25-hydroxylase

Recombinant Vitamin D (3) 25-hydroxylase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP523701PIYeast1mgQuote
EP523701PIE. coli1mgQuote
BP523701PIBaculovirus200ugQuote
MP523701PIMammalian Cell200ugQuote

Protein Information

SpeciesSus scrofa (Pig)
UniProt IDO46658
Gene NameCYP2D25
Protein NameVitamin D (3) 25-hydroxylase
Region Expressed2-500
Expression Tag6xHis
Purity>90%
AA SequenceGLLTGDLLGILALAMVIFLLLVDLMHRRSRWAPRYPPGPMPLPGLGNLLQVNFQDPRLSF IQLRRRFGDVFSLQQIWRPVVVLNGLAAVREALVSHSHETSDRPPVFILEHLGYGPRSEG VILARYGKAWREQRRFSVSTLRNFGLGKKSLEEWVTQEASCLCAAFADQAGRPFSPNNLL NKAVSNVIASLTFARRFEYNDPRMLKLLDLVLEGLKEEVGLMRQVLEAMPVLRHIPGLCA KLFPRQKAFLVMIDELITEHKMTRDLAQPPRDLTDAFLDEMKEAKGNPESSFNDENLRLV VAHLFSAGMITTSTTLAWALLLMILHPDVQRRVQQEIDEVIGHVRQPEIKDQALMPFTLA VLHEVQRFGDIVPLGVAHMTSCDIEVQGFLIPKGTTLITNLTSVLKDETVWKKPFRFYPE HFLDAQGRFTKQEAFMPFSAGRRSCLGEPLARMELFLFFTTLLQAFSFSVPTGQPCPSDH GVFAFLLFPSPYQLCAVPR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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