Request QuoteCatalog Number: xP517472VEXSize: 0.2-1mg

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Recombinant Vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase (vibE)

Recombinant Vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase (vibE) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP517472VEXYeast1mgQuote
EP517472VEXE. coli1mgQuote
BP517472VEXBaculovirus200ugQuote
MP517472VEXMammalian Cell200ugQuote

Protein Information

SpeciesVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
UniProt IDO07899
Gene NamevibE; Locus:VC_0772
Protein NameVibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase
Region Expressed1-543
Expression Tag6xHis
Purity>90%
AA SequenceMTTDFTPWPEALAAQYRQLGYWQDKTLLDYLQQSAERTPNALALVGDNQQWRYQAMLERI EQLAAGFTELGLGCGDNVVLQLGNVAEFYLCFFALLRQGIRPILALPAHRLAEIRYFCQH SQAKAYLIDGAQRPFDYQALAQELLACCPTLQTVIVRGQTRVTDPKFIELASCYSASSCQ ANADPNQIAFFQLSGGTTGTPKLIPRTHNDYAYSVTASVEICRFDQHTRYLCVLPAAHNF PLSSPGALGVFWAGGCVVLSQDASPQHAFKLIEQHKITVTALVPPLALLWMDHAEKSTYD LSSLHFVQVGGAKFSEAAARRLPKALGCQLQQVFGMAEGLVNYTRLDDSAELIATTQGRP ISAHDQLLVVDEQGQPVASGEEGYLLTQGPYTIRGYYRADQHNQRAFNAQGFYITGDKVK LSSEGYVIVTGRAKDQINRGGEKIAAEEVENQLLHHPAVHDAALIAISDEYLGERSCAVI VLKPEQSVNTIQLKRFLHQAGLADYKIPDQIQFIDQLPKTSVGKIDKNALRRRFDTLGLA LMS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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