$148.20Catalog Number: MAB316-20ugSize: 20ug

VHL Antibody

Von Hippel-Lindau Protein, Mouse Anti Human
Monoclonal Antibody

Monoclonal antibodies provide the best target specificity and affinity. It is also advantagous for long term projects as there is little lot to lot variation, where polycolonal antibodies will vary significantly with different preparations due to immunogen preparation and animal responses.

Applications

VHL antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.

Clone

P52A11AT.

Ig Subclass

Mouse IgG2b heavy chain and κ light chain.

Immunogen

Anti-human VHL mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human VHL amino acids 1-154 purified from E. coli.

Protein formulation

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

Purification Method

VHL antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

Stability / Shelf Life

12 months at -20oC. 1 month at 4oC.

Storage Procedures

For periods up to 1 month store at 4oC, for longer periods of time, store at -20oC. Prevent freeze thaw cycles.

Synonyms

Von Hippel-Lindau disease tumor suppressor, pVHL, Protein G7, VHL, RCA1, VHL1, HRCA1.

Type

Mouse Anti Human Monoclonal.

Quantity

$148.20

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