Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells (PAE/FLT -4 cells). The ED50 for this effect is typically 150-300 ng/ml, corresponding to a specific activity of 3.3-6.6KUnits/mg.
The protein was lyophilized from a concentrated (1mg/ml) solution with BSA.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor C 152 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Vascular Endothelial Growth Factor-C152 although stable at room temperature for 3 weeks, should be stored desiccated below
-18oC. Upon reconstitution VEGF-C 152 should be stored at 4oC between 2-7 days and for future use below -18oC.
Please prevent freeze-thaw cycles.
VEGF-C152, Vascular endothelial growth factor C 152, VRP, Flt4 ligand, Flt4-L.
