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Recombinant Valine--tRNA ligase (valS)

Recombinant Valine--tRNA ligase (valS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP412368NFAYeast1mgQuote
EP412368NFAE. coli1mgQuote
BP412368NFABaculovirus200ugQuote
MP412368NFAMammalian Cell200ugQuote

Protein Information

SpeciesNitratiruptor sp. (strain SB155-2)
UniProt IDA6Q3R4
Gene NamevalS; Locus:NIS_1013
Protein NameValine--tRNA ligase
Region Expressed1-871
Expression Tag6xHis
Purity>90%
AA SequenceMSKATKYDPKKVERQFYQIWETRGYFETDGNKKIQNGKTFCIMMPPPNVTGRLHIGHALT FTLQDIMVRYKRMDGYETLWQPGTDHAGIATQNVVEKQLLSKGIKKEEIGREKFLEYVWK WKEESGNAIVTQLRLLGVSPAWSRERFTMDKGLKNAVREAFVNLYYEGLIVKGNYMINWC THDGALSDIEVEYEEKEGALYHIKYPIVGSDEYLVVATTRPETYFGDTAVMVNPNDERYK HLIGKKVRLPLINREIPIIADEHVDMEFGTGAVKVTPAHDPNDYEVGKRHNLPFITIFDE NGILNEEAGEFAGIERLEARKKVVEKLEQEGFIEKIEPHKHQVGHCYRCGNVVEPYISPQ WFVKAEIAKEAVKKANEGETKFYPPQWLNNFNAWMRELRDWCISRQLWWGHRIPVWYCRA CGHEWASKKEHEESCPKCGSTDIYQDPDVLDTWFSSALWPFSTLGWGNGDWGKGVKWFED DLKKFYPNDLLITGFDILFFWVARMMMMGEHFLHKLPFKDVYLHALVRDEHGQKMSKSRG NVIDPIDTIEEYSADALRFTLAALAVQGRDIRLSKERLELYRNFTNKLYNAARFLQIHQE KFDDLQNIQIKTDLGKYILSRFGLAIQEVRNNLNSYRFNDAATTLYRFLWGEFCDWGIEL SKVNKDAIAELGAIFKESMKLLHPFMPFITEFLYQELSGTSIEENESIMIQPYPKAAPID EEIMKRFETIIDAIVSIRRAKALIDMANKTIPKVLIKGDLEESAKAYISKLAKVETIEFV SEPAENAVTDIGNYVEVFIPLEGIDLTPILNRLNKQKEKLQKEIDKLSRMLSNENFVKNA PQAVVEQNRAALAEAQNRLATIEEELARLTR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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