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Recombinant Valine--tRNA ligase (valS)

Recombinant Valine--tRNA ligase (valS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP500043LNOYeast1mgQuote
EP500043LNOE. coli1mgQuote
BP500043LNOBaculovirus200ugQuote
MP500043LNOMammalian Cell200ugQuote

Protein Information

SpeciesLaribacter hongkongensis (strain HLHK9)
UniProt IDC1D6J9
Gene NamevalS; Locus:LHK_01113
Protein NameValine--tRNA ligase
Region Expressed1-937
Expression Tag6xHis
Purity>90%
AA SequenceMELAKSFEPGNIENRWYDRWEAAGYFKPSMDTDRPSFCIQLPPPNVTGTLHMGHAFNQTI MDGLTRYYRMKGDNTLWVPGADHAGIATQIVVERQLAEQGVSRHDLGRDTFIGKVWEWKE KSGGTITSQMRRVGCSVDWDKEYFTMDDKMSTAVTEVFVRLYEQGLIYRGKRLVNWDPKL GTAVSDLEVISEEEDGKMWHIRYPVVGSDDVVVVATTRPETLLGDVAVAVNPDDERYRHL VGKQLELPLTGRTIPVIADDYVDAAFGTGFVKITPAHDFNDYQVGKRHNTALINVMSLDA TMLAKAQVFGFDGSAQGSIDLPAAYAGLSTADARKAMLADLDAAGLLVDTKPHKLMVPRG DRTGSVIEPLLTDQWFVAMTKVGEGDATGKSITQKAIDAVESGEVRFVPENWVNTYNQWM NNIQDWCISRQLWWGHQIPAWYDEDGKAYVGRTLEEVQAKAPGKTLRRDEDVLDTWFSSA LVPFSSLGWPNETPELKAFVPSQVLVTGYEIIFFWVARMIMMTTHFLGKVPFKDVYIHGI VRDHEGKKMSKSEGNVIDPVDLIDGIALPELITKRTTGLRRPEKAPQIVKATEKLFPEGI PAYGTDALRFTMASYASLGRSVNFDFKRAEGYRNFCNKLWNATRFVMMNVEGKDCGQDES LPLEYSFVDKWIISRLQELEAAVTEALDTYRFDMASQLIYEFVWNEYCDWYVELAKVQLA NGNEAQQRATRRTLVRVLEVALRLTHPLMPFITEELWQTVAPLANAKKTDSIMVAAWPVA DMSKVDAEACGRMTVFKELVNAIRNLRGEMNLGPSVKAPLFVEGPAAYADFLPYARLLGR LSDAAVVEKLPDADAPVAIAGEARLMLKVEIDKAAETARLTKEIGKAESDVEKLTAKLEK PGYVDKAPAQLVERDRAQLADLTDKLAKLKAQLLKLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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