Request QuoteCatalog Number: xP517939MSRSize: 0.2-1mg

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Recombinant Valine--tRNA ligase (valS)

Recombinant Valine--tRNA ligase (valS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP517939MSRYeast1mgQuote
EP517939MSRE. coli1mgQuote
BP517939MSRBaculovirus200ugQuote
MP517939MSRMammalian Cell200ugQuote

Protein Information

SpeciesMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
UniProt IDO26861
Gene NamevalS; Locus:MTH_767
Protein NameValine--tRNA ligase
Region Expressed1-877
Expression Tag6xHis
Purity>90%
AA SequenceMTDNQIPKDYNHKNEVKWQKKWQEEDIYRFIGSGTKPRYIIDTPPPYPTGSIHMGHVLNW VYMDIIARFKRMRGFDVLFPQGWDCHGLPTEVKVEETHNIKKSDVSREEFRRLCVELTQE NIRMMKEQMQRLGFSQDWNHEFVTMTPEYMRRTQLSFLRMYRDGLIYQGVHPVNWCPRCE TAIAFAEVEYIENETNLNYVRFPVEGADEHITIATTRPELMAACVAVVVHPDDERFREFE DKLIEVPLFGQKVKLIKDPEVDPEFGTGAVMVCTFGDKTDVSWVNRHGLDVIDAIDERGY MTEAAGKYQGLTIAECKEKIVEDLENEGFLLKKEPVRQNVGTCWRCKTPIEILVKKQWFV AVKKLIPQVREAAEEMKWVPGHMKTRLLNWTGSMDWDWCISRQRIFATPIPVWYCSECGR VHVADEEMLPVDPTRDGPGITCECGSTEFIGEEDVLDTWMDSSISPLSVAGWPDESYREL FPADLRPQGHDIIRTWAFYTILRCMALTGEKPFSEIVINGMVFGEDGHKMSKSRGNVIAP EEVLEDYGADALRLWAAGSVPGSDVPFAWKDVKYGYKFLRKFWNAFRFISIHLTENPEVE ARPMDRWILSRLMNLVAEVTESLDDYNFAAAVNRVQTFIWHDFCDEYIEAVKYRLYSDED PESRMAAQNTLRMVLDTCLRLLAPVAPHFTEEVHQHVGEGSIHLRGWPEHIPEIVDPEIE RSGDLAVEIIGEIRRFKSSSKMPLNAPLKAATIYTDNESAEMIKPFLDDIAGTMNIGDIS LVAGKPEITERAVELEPRMEKIGPEFRSDAPAIISWLTGADPHEVYEEIQRNGEIEVEGN RLTMDHISFRKEVIGTAGERVDVLNLDEPEVIIEIVR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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