Request QuoteCatalog Number: xP503776RMNSize: 0.2-1mg

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Recombinant Valine--tRNA ligase (valS)

Recombinant Valine--tRNA ligase (valS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP503776RMNYeast1mgQuote
EP503776RMNE. coli1mgQuote
BP503776RMNBaculovirus200ugQuote
MP503776RMNMammalian Cell200ugQuote

Protein Information

SpeciesRickettsia africae (strain ESF-5)
UniProt IDC3PLG3
Gene NamevalS; Locus:RAF_ORF0957
Protein NameValine--tRNA ligase
Region Expressed1-812
Expression Tag6xHis
Purity>90%
AA SequenceMKEFPKNYNFTENEKKWQQIWQEKQIYAYNPNVAKEETYVIDTPPPTVSGQLHIGHVYSY TQTDFIVRFQRMMGKNIFYPMGFDDNGLPTERLVEKQKQIKAYNMDRSEFIKICEEVVES EEEKFRSLFNQIALSVDWSLEYQTISPLSRKISQMSFLDLVQKEEIYRTNQPILWDPVDG TALAQADIEDKEKTSFMNYITFKTAQGDPLTIATTRPELLPACVAVFYHPDDGRYKHLAG KSAITPLFNEQVPLLADPLVRQDKGTGLVMCCTFGDQTDITWWKTHNLPLKTIITKKGTI DFQHETSIDGLKIKEARTKIIDILKEQELLIKQEDITHTVKCAERSGAPLEILTVPQWFV KTISHKEELLKRANELNWHPKNMKIRLENWINAISWDWCISRQRYFGVPFPVWYSKRVGE EGKILYADVTQLPIDPLKDLPVGYSKEEVEPDYDVMDTWATSSVSPQLSTHGISDDFAVN KDRHDKLFPMDLRPQAHEIIRTWAFYTILKAHLHQNTLPWKNIMVNGWCLAEDRSKMSKS KGNVLVPEKLLEQYGSDVIRYWSANSKLGADTTYSEDVMKNGKRLVNKLWNAAKFVFIHF DKLKGEDKKASLLDIKEKITNEFDKWMVNKLVELVKLATNELQNYEYANAMHLTEKFFWV VLCDNYLEISKTRSYDEEHKNPQGQYSSILTLYHVMQTLLKLFAPFMPHITEELYQILYS ENSIHVKGSWVNYSDLNYEINAKGAEGLLEILDIVRKFKAEKNLSIKTPIKLLEVSGIVL SAELAEDLKNVTSAEEIQFEMKDDKIKVNIIL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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