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Recombinant Valine--tRNA ligase (valS)

Recombinant Valine--tRNA ligase (valS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP461006BTAYeast1mgQuote
EP461006BTAE. coli1mgQuote
BP461006BTABaculovirus200ugQuote
MP461006BTAMammalian Cell200ugQuote

Protein Information

SpeciesBifidobacterium longum (strain DJO10A)
UniProt IDB3DU23
Gene NamevalS; Locus:BLD_1197
Protein Name
Region Expressed1-911
Expression Tag6xHis
Purity>90%
AA SequenceMTEGKSIINANLTPLPDKVGVDGLEDKWRAVWDEDGTYKFRNTRDRKAVYSIDTPPPTVS GSLHVGHVFSYTHTDVIARYKRMRGYDVFYPMGWDDNGLPTERRVQNYYGVRVDVSLPYD PDFKPPFEGTDGKKIDAKDQVPISRKNFIELCERLTAQDEKLFEALWRKLGLSIDWSQTY HTIGQHPQRVAQKAFLRNLARGEAYQQDAPGLWDVTFQTAVAQAELESREYPGFYHKVAF RFEDGTPIYIETTRPELLAACTSLIANPNDERYKQYFGQYVYSPLFKVKVPILAHPAAEM DKGAGIAMCCTFGDVTDVEWWRDLKLPTRPIIQRNGRIVMDTPDWIEDPAGREVFAETAG KTTFSARKIIVDKLRESGDLDGEPTPTKRMTNFYEKGDKPLEIVTSRQWYLKNGGTDAKL NAELIERGKELEFHPDFMRVRYENWVHGLNGDWLISRQRFFGVPFPLWYPVNASGEPDYD HPITPSEDRLPIDPTIDVPEGYDESQRDVPGGFTAEKDIMDTWATSSLTPQIVTHWAEPD EASKALFASTFPMDLRPQGQDIIRTWLFSTVDRAHLENKCLPWAHATLSGWILDPDHKKM SKSKGNVVVPNEPIEKFGADAVRYWAAAARLGLDATYDIGQMKIGRRLAIKLLNATKFAL AIGREDENHHVGAAAEAAWNPADVTEPLDRAAMAKLALVVRQATEALESYEHSKALEVIE SYFWQFCDDYIELVKNRAYGTPDEHGNVPSEKAVKSARTALGLGLDAFARLLAPYLPYAT EEVWSWMHAGSGSVHRTAWPVVDPYVEAATGASPELLTWAGKAVEQLRKIKSEAKVSMKT PILSVALSAASEGVEAIHAALGDIAQAGRVVGKFDLVAKHAEESAAEDAPETEVAVEASE LGEPPAKKPKH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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