Request QuoteCatalog Number: xP424718RILSize: 0.2-1mg

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Recombinant Valine--tRNA ligase (valS)

Recombinant Valine--tRNA ligase (valS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP424718RILYeast1mgQuote
EP424718RILE. coli1mgQuote
BP424718RILBaculovirus200ugQuote
MP424718RILMammalian Cell200ugQuote

Protein Information

SpeciesRickettsia akari (strain Hartford)
UniProt IDA8GPJ3
Gene NamevalS; Locus:A1C_05365
Protein NameValine--tRNA ligase
Region Expressed1-812
Expression Tag6xHis
Purity>90%
AA SequenceMTEFPKNYNFTENEKKWQQIWQAQQIYAYDPNISKEETYVVDTPPPTVSGQLHIGHVYSY TQTDFIVRFQRMIGKNIFYPMGFDDNGLPTERLVEKQKQIKAYNMGRDEFIKICEEVVES EEEKFRRLFNQIALSVDWSLEYQTISPLSRKISQMSFLDLVKKGKIYRNDQPILWDPVDG TALAQADIEDKAKTSFMNYIIFKTEQGESLTIATTRPELLPACIAVFYHPDDKRYKHLAC KSAITPLFNEKVPLLASPLVQQDKGTGLVMCCTFGDQTDITWWKTHNLPLKTIITKKGTI NFPHNIAIDGLKIKEARIKIIDILKEQKLITKQEEITQTVKCAERSGAPLEILTVPQWFV KTISHKEALLKRANELNWRPNNMKIRLENWINAISWDWCISRQRYFGVPFPVWYSKRVGE EGKILYADISQLPVDPLKDLPIGYSKEEVEPDLDVMDTWATSSVSPQLSTHCISDNFAVN KDRHNKLFPMDLRPQAHEIIRTWAFYTILKAHLHQNTLPWGNIMVSGWCLAEDRSKMSKS KGNVLVPEKLLEQYGSDVIRYWSANSKLGADTAYSEDVMKNGKRLVNKLWNACKFIFIHC NKLKDVDKKASLFDIKEQITNEFDQWMINKLVELVNAATNELQNYEYANAMHLTEKFFWV IFCDNYLEISKTRSYDEENKNPQGQYSSILTLYHVMQILLKLFAPFIPHITEELYQILYS ENSIHVKGNWVNYGDLHYGIDVTQSEGLIAILDIVRKFKAENNLSIKAPIKLLEVSGIEL SAELTEDLKNVTSAEEIQFEGQGDKIKVNVIF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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