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Recombinant tRNA-splicing ligase RtcB homolog (MICPUCDRAFT_24370)

Recombinant tRNA-splicing ligase RtcB homolog (MICPUCDRAFT_24370) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP003810MTTYeast1mgQuote
EP003810MTTE. coli1mgQuote
BP003810MTTBaculovirus200ugQuote
MP003810MTTMammalian Cell200ugQuote

Protein Information

SpeciesMicromonas pusilla (strain CCMP1545) (Picoplanktonic green alga)
UniProt IDC1MI97
Gene NameORFs:MICPUCDRAFT_24370
Protein NametRNA-splicing ligase RtcB homolog
Region Expressed1-507
Expression Tag6xHis
Purity>90%
AA SequenceMAPRRTYQQEMAFLSRVSANQWIVNEGFVPNMRVPGTFYVNKHLETLMFEELQQHVDSGG HGGFLPAVKQLANVACLPGIVGKSIAMPDVHSGYGFCIGNVAAFDMSDPAAVVSPGGVGF DINCGVRVVRTNLMESDVADVKEKLAQALFDHIPVGVGSQGIIPTSAAGLESALEMGMDW SLREGYAWAEDKEHCEEYGRMLTADPNKVSARAKKRGLPQMGTLGAGNHYAEIQVVDEIF DAHAAEKMGVDAVGQVMVMIHSGSRGLGHQVATDALTEMERAMARDGIETNDRQLACARI NSTEGQNYLSAMSCAANYAWVNRSSMTFLCRQAFAKMFDSTPDDLDMHVVYDVSHNIAKI EEHMVDGKLKTLLVHRKGSTRAFPPHHPLIPVDYQYTGQPVMIGGTMGTCSYILTGTQKG MDETFGSTCHGAGRARSRNNSRNKLDYAEVLENLKTKGISIRVASPKLVMEEAPESYKDV TEVVNTCHDAGISKKAVKLRPIAVVKG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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