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Recombinant tRNA-splicing ligase RtcB homolog (PKH_090290)

Recombinant tRNA-splicing ligase RtcB homolog (PKH_090290) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP003810EWUYeast1mgQuote
EP003810EWUE. coli1mgQuote
BP003810EWUBaculovirus200ugQuote
MP003810EWUMammalian Cell200ugQuote

Protein Information

SpeciesPlasmodium knowlesi (strain H)
UniProt IDB3L4K9
Gene NameORFs:PKH_090290
Protein NametRNA-splicing ligase RtcB homolog
Region Expressed1-511
Expression Tag6xHis
Purity>90%
AA SequenceMKQGSRSEGARGDIRGYIQKTGERNLYRIKKGLVPNMNVEGHMYVNDKLKHLIDDEIEMY QLNRNSTFLPAVVQIANVSTLPGIVKASIALPDVHAGYGFSIGNVAAFDMSNEKAIISPG GVGFDINCGVRLIRTNLFYEDIKDKQDELAQLLFNNIPVGVGSQGCILCNQDKLDEALCL GMDWCVKEGYAWVEDKLNCEDNGRSFYADSNYVSVRAKKRGITQMGTLGAGNHYAEIQIV DEIYDKRSAKLMGIEKKNQVCIMIHSGSRGLGHQIATDALIEMEKSMTKYKIDVIDKQLA CTPIHSKEGQNYLKAMGSACNFAWINRSSMTFLARQTFAKIFNQSPDDLDMHVIYDISHN IAKMEDHLVDGKMKKLLVHRKGSTRAFPPFHPAVPLDYQYCGQPILIGGTMGTYSYVLTG TDKAMETTFGSTCHGAGRALSRNKSRNTLNYMDVLQKMKEENISIRVASPKLIMEEAPES YKNVSEVVNTCHEAGISNKCFRLKPVAVIKG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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