Request QuoteCatalog Number: xP518715VFSSize: 0.2-1mg

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Recombinant tRNA pseudouridine synthase Pus10 (pus10)

Recombinant tRNA pseudouridine synthase Pus10 (pus10) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP518715VFSYeast1mgQuote
EP518715VFSE. coli1mgQuote
BP518715VFSBaculovirus200ugQuote
MP518715VFSMammalian Cell200ugQuote

Protein Information

SpeciesVulcanisaeta distributa (strain DSM 14429 / JCM 11212 / NBRC 100878 / IC-017)
UniProt IDE1QUT8
Gene Namepus10; Locus:Vdis_0543
Protein NametRNA pseudouridine synthase Pus10
Region Expressed1-412
Expression Tag6xHis
Purity>90%
AA SequenceMSIERAMDILRKYPLCNHCLGRLFARLGTGLDNEERGRAIKDYLLMRIHERILNEGLSDE LLNDVKALAVSGHEPSIKFLSNMGINVSPARCYVCGDTIFNRLNEWVNNIVNSLRSLGIE FRSFRLGSRVPLDIQNRELSITTEFNISSAESIKREINRELGKRVSAMLGVSFNREEPDV EVVIDVNTGSVEVQIMPIYISARYRKVHRLINEEGQVKWPIDRVIQAYNAQDVVIHTGGE DPMGVRVLGNGRPVILQVVKPSKRPDVNDVYALLKDSGYDIVLDNLSRVRASAVVKMKAR VRDYVITYRVLAITDNSVTNENIKSLHDYFRNRQVVQVFRRGRRIRRRISMVYELDGRVI RDRLVEFLIRCQGNLYIRGFVHGGLGDVEPSIAGTLGFSVRPVEIDILNISD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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