Request QuoteCatalog Number: xP516722ILYSize: 0.2-1mg

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Recombinant tRNA pseudouridine synthase Pus10 (pus10)

Recombinant tRNA pseudouridine synthase Pus10 (pus10) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP516722ILYYeast1mgQuote
EP516722ILYE. coli1mgQuote
BP516722ILYBaculovirus200ugQuote
MP516722ILYMammalian Cell200ugQuote

Protein Information

SpeciesIgnisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)
UniProt IDE0SNQ8
Gene Namepus10; Locus:Igag_1040
Protein NametRNA pseudouridine synthase Pus10
Region Expressed1-430
Expression Tag6xHis
Purity>90%
AA SequenceMSIIDIANNILRKYCLCDRCLGRLFASLGRGLSNDERGKAIKIALVLELHKRYLDGDKDS LKQLVELSPNIGPIAVNLIKNLGIEIEYTPRTCFICDNKINDIIDTYSQRIADIINERHI NSFVLGIRGVTSYIRKEESIANEFKLLYWENIKRELKREIGKKIQMMTNAKVDFLNPEAM IIIDIDRDRIYIESPSLLIYGRYWKLGRMISQNIWLTKNGVKKYPLSIEEIAKMLVKDGF GDDVVLHIAGREDVDVRTLGSGRPFVLEIKRPRKRNIDIKDIENRLNSISRWLKFELNMF VDRDFVSRVKKGCRTSYKIYRAIVVADRDIGIEDIKRLEEFFRDRIIEQRTPTRVLRRKK DVLRRRKVFEIKTRVISPRVFEALIKCEGGLYVKELISGDNGRTVPSFSSVLNANTLCLT LDALYVHEYI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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