Request QuoteCatalog Number: xP520439AWBSize: 0.2-1mg

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Recombinant tRNA pseudouridine synthase Pus10 (pus10)

Recombinant tRNA pseudouridine synthase Pus10 (pus10) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP520439AWBYeast1mgQuote
EP520439AWBE. coli1mgQuote
BP520439AWBBaculovirus200ugQuote
MP520439AWBMammalian Cell200ugQuote

Protein Information

SpeciesAcidilobus saccharovorans (strain DSM 16705 / VKM B-2471 / 345-15)
UniProt IDD9PZU4
Gene Namepus10; Locus:ASAC_0174
Protein NametRNA pseudouridine synthase Pus10
Region Expressed1-400
Expression Tag6xHis
Purity>90%
AA SequenceMNEDPAQQAMKVLAKYPLCDRCLGRLFAGLGRGLSNAERGRALKLSVVMSLHAMSLSGRL PENARDVLANAGGAAAQVYVELFGSPPEHRSCYICNDALDKFLDEMPQRVADAVKEWGGS TFLVGAKVDPGVIAREERIKAEFGLAFGESIKSEIKRELGKRAQQLGPKVSFDRPDVVVM VSFPDGSVSVQPRRLVVKGLYRRLRRDLQLRPREALSHPLVARLINDTRSSRVGIVGLVR DEKEVRALGLGIPVEFHLGGPRVRLVPPDGSIIEAEGASLEVNSSVEASGPEDLSRRVRV YRCVLYVEGGAFESLQLAAASLRGKEVRQKFMGKEVSGVVRGAECVDMGGGLAECIIALD ERLHVMELVSGSGTEPSLSGLLGTAAECVVADLLGVIPLR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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