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Recombinant tRNA pseudouridine synthase Pus10 (pus10)

Recombinant tRNA pseudouridine synthase Pus10 (pus10) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP518693MSEYeast1mgQuote
EP518693MSEE. coli1mgQuote
BP518693MSEBaculovirus200ugQuote
MP518693MSEMammalian Cell200ugQuote

Protein Information

SpeciesMethanococcus voltae (strain ATCC BAA-1334 / A3)
UniProt IDD7DQH5
Gene Namepus10; Locus:Mvol_1447
Protein NametRNA pseudouridine synthase Pus10
Region Expressed1-519
Expression Tag6xHis
Purity>90%
AA SequenceMDYENKILQKYNLCNRCYGRIYAKLMRMGNKDRGTSIKTVIAMNFEEKLKDLIEEKNNLI DIISNSQDINENEEIDENTKNNEDTENKADDKSQSNEEKIQEIDEKINIIKENLTLLRKT GLNGIKNEYIIEELLKDDITREENESEENESNIFLTPEQKCPWCKDVFNIQNLEEIADKI VEALSEYEFDRFLIGTRLPKRIKELEKDLETTFNEKNTESLRNEFGRELGKILTKKLEKP VDKETPDIVVMVNPYNQKIYLQINPIFIKGRYRKTKRGIPQSHWDCRSCRGKGCEKCNFT GKQYPTSVEEIIAEPVMNIAKGSGEALHAAGREDIDVKMLGKGRPFVIEVKEPKVRKMDL LKIMDEINKIEGVEVSDLEYGVKNDVRFFKNEPHTKTYCALVSIVDEELENHDFENDKIV ELSEKLENLRIDQRTPHRVSHRRADLVRVRNIYKAWCEPIDEKSFKLTVYCDGGLYIKEL ISGDEGRTKPSISEILDIPCYCKLLTVMEVHDENNPANY
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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