Request QuoteCatalog Number: xP514944NFSSize: 0.2-1mg

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Recombinant tRNA (guanine (37) -N1) -methyltransferase

Recombinant tRNA (guanine (37) -N1) -methyltransferase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP514944NFSYeast1mgQuote
EP514944NFSE. coli1mgQuote
BP514944NFSBaculovirus200ugQuote
MP514944NFSMammalian Cell200ugQuote

Protein Information

SpeciesNectria haematococca (strain 77-13-4 / ATCC MYA-4622 / FGSC 9596 / MPVI) (Fusarium solani subsp. pisi)
UniProt IDC7YK87
Gene NameTRM5; ORFs:NECHADRAFT_98685
Protein NametRNA (guanine (37) -N1) -methyltransferase
Region Expressed21-465
Expression Tag6xHis
Purity>90%
AA SequenceAKTLDRSVFAKTLNAAAASISENRLLSKYRKELEKTQEVLFMERFNPILPDPDPSLASQG RKCIVLSPQIKPASPETWSPTLQEASKLGELKVVPYDLEITYDFWNYFDVVKSILPEELH GEIPSGFNTVGHVAHLNIRDQYLPYKNIIAQVLLDKNPHIKTVINKIDNVGSENEFRTFA YEVLGGPDNMNVEVSEAGCIFRFDYSKVYWNSKLDTEHKRITSFFKPGEVVADVMAGIGP FAVPAGKKGVFVWANDKNPESHRYLEDAIQKNKVWEFVKPFNHDGHDFIRTSADLVLEAS KRGDCAVIKPPRPPRKSAAPPPEPVRVPVPPTISHFVMNLPASAIEFLHNYRGLYHGHED LFEPHTETKLPIVHVHCFSAKMDDDTPLKDICERIHKEIGVMLKPGDAEKEGEVLIYDVR DVAPAKRMFCASFRLPREVAFAARA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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