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Recombinant tRNA (guanine (37) -N1) -methyltransferase

Recombinant tRNA (guanine (37) -N1) -methyltransferase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP519003MOWYeast1mgQuote
EP519003MOWE. coli1mgQuote
BP519003MOWBaculovirus200ugQuote
MP519003MOWMammalian Cell200ugQuote

Protein Information

SpeciesMacaca mulatta (Rhesus macaque)
UniProt IDF7GSQ4
Gene NameTRMT5; aka: TRM5
Protein NametRNA (guanine (37) -N1) -methyltransferase
Region Expressed58-509
Expression Tag6xHis
Purity>90%
AA SequenceSTMPEIETHERDSELFSPPSDVRGMTKLDRTAFKKTVNIPVLKVRKEIVSRLMRSLRRAA LQRPGIKRVIEDPEDKESRLILLDPYKIFTHDSFEKAELSVLEQLNVSPQISKYNLELTY ENFKSEEILRAVLPEGQDVTSGFSRVGHIAHLNLRDHQLPFKQLIGQVMIDKNPGITSAV NKINNIDNMYRNFHMEVLSGEQNMMTKVRENKYTYEFDFSKVYWNPRLSTEHSRITELLK AGDVLFDVFAGVGPFAIPVAKKNCTVFANDLNPESHKWLLHNCKLNKVDQKVKIFNLDGK DFLQGPVKEELIQLLSLSKERKPSVHIVMNLPAKAIEFLSAFKWLLDGQPCSNEFLPIVH CYSFSKDANPAKDVRQRAGAVLGISLEACSSVHLVRNVAPNKEMLCITFQIPAAVLYKNQ TKNPENHEDPPLKRQRTAEAFSDEKTQIASNT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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