Request QuoteCatalog Number: xP515276DTLSize: 0.2-1mg

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Recombinant tRNA (guanine (37) -N1) -methyltransferase

Recombinant tRNA (guanine (37) -N1) -methyltransferase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP515276DTLYeast1mgQuote
EP515276DTLE. coli1mgQuote
BP515276DTLBaculovirus200ugQuote
MP515276DTLMammalian Cell200ugQuote

Protein Information

SpeciesCiona intestinalis (Transparent sea squirt) (Ascidia intestinalis)
UniProt IDF7A355
Gene Name
Protein NametRNA (guanine (37) -N1) -methyltransferase
Region Expressed1-456
Expression Tag6xHis
Purity>90%
AA SequenceMVIAYIRNLIRKVIQRGMDHEHSALYPPAAVRGMKVLDRSAFEQEVTLPALLVPVQSLRN CRKMMKSKLVNLSINKKVVDPPAHLVGEELTAGHKLFLLKPGTTMETYSSDEKQMLGKLG VKDKIYDYRITLGYENYKHWDVLRAILPDSEMAARGFSQVGHILHVNLRDHQLPYKHIIG QVLLDKIQTARTVVNKHQNIDNKFRNFEMEVIAGENNFVTRIIEHGRKFEFDFSKVFWNS RLSTEHQRITNFVSESDVVFDVFAGVGPFAIPIAKKGCVVYANDLNPESYRWLLHNVALN KTKAKCFNSDGREFIQTELRNYLLTRSPHKVHVLMNLPAIAVEFLDVFRGLLCNGDCLAA NDLSNASHKKLLSIPEVCVHLYIFAPEKDGIADLKSRIKQSLGCALPEDAVIYNVRNVAP KKQMYCVSFVLSQHWMDQKEQDVYEPAPKKIKECIS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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