Request QuoteCatalog Number: xP515275XBFSize: 0.2-1mg

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Recombinant tRNA (guanine (37) -N1) -methyltransferase

Recombinant tRNA (guanine (37) -N1) -methyltransferase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP515275XBFYeast1mgQuote
EP515275XBFE. coli1mgQuote
BP515275XBFBaculovirus200ugQuote
MP515275XBFMammalian Cell200ugQuote

Protein Information

SpeciesXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
UniProt IDF6VSS6
Gene Nametrmt5; aka: trm5
Protein NametRNA (guanine (37) -N1) -methyltransferase
Region Expressed33-494
Expression Tag6xHis
Purity>90%
AA SequenceHQLPPTISATRSCFSAMAEMQDSHNEMELYSPNPEVRGMTCLNRDAFDKTIHVPVIKIKK EIINRLMKSLKHRLIQRPSLKRVIEDPKDDVNKLVLLDPYKVKSIDSFDESDHVLFKQFD VNPQVSQYELQLTYENFKCEEILRAVLPKGQDVTSGFSRVGHIAHMNLRDHQLPYKNVIG QVILDKNPGITSVVNKTNTIDSTYRNFQMEVLAGEENMITKVKENYVTYEFDFSKVYWNP RLGTEHNRIIGFLKARDVLFDVFAGVGPFAVPAAKKNCTVYANDLNPESYKWLLHNCKLN KVEKRVQAFNTDGRDFIKTTIKKELLKYADMPSAEEKPSLHIAMNLPALAVEFLDAFKNL LEEEPCNSFILPTIHCYSFSKDDDPLQDVKARAESFLGTTLEDCSMHLVRNVAPNKEMVC ISFKLPSSVLFQRLSDTGEPESKRPRTAEAFPLPHVQQSRNS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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