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Recombinant tRNA (guanine (37) -N1) -methyltransferase

Recombinant tRNA (guanine (37) -N1) -methyltransferase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP518992BSIYeast1mgQuote
EP518992BSIE. coli1mgQuote
BP518992BSIBaculovirus200ugQuote
MP518992BSIMammalian Cell200ugQuote

Protein Information

SpeciesBatrachochytrium dendrobatidis (strain JAM81 / FGSC 10211) (Frog chytrid fungus)
UniProt IDF4NUJ6
Gene NameTRM5; ORFs:BATDEDRAFT_85132
Protein NametRNA (guanine (37) -N1) -methyltransferase
Region Expressed1-475
Expression Tag6xHis
Purity>90%
AA SequenceMKYIFAPPANKGLQQLNHALFESSHTLPALKIPAHASGIAMETLRSHILVAPRLRTIVDD PTDKKWRLLLLDPSMEASEINDLPNPLKEFALKHEAKLVKHTIELKYDYWTSDQVLRSIL PDEMETPGAFETVGHIAHLNLRDRYQPFKHIIGQVILDKSSHIKTVVNKLDNIDHTFRFF QMEILAGINDMNAKLKEGGCFFHFDFSKVYWNSRLQGEHDRIIKLFGQNDLICDVFAGVG PFALPAAKHKRCVVFANDLNPQSFKYLMENIKLNKLETRILPFNMDGRQFIKQSLEDLNN PAIWNKITKQKPTSNDKKRNRKVESPTVAPLTDQPAISGIRHFKHYVMNLPATAIEFLDA FHGLYSGMRDVIMDSDLPTIHCHCFSNAKDVKADVIERVERVIGMPLGSNLIMVHSVRTV APNKDMLCISFRLPSALAFAEPKILGKRKGLETEENLVSQSDVSKSSDNILEKDT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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