Request QuoteCatalog Number: xP520650FHISize: 0.2-1mg

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Recombinant tRNA (guanine (37) -N1) -methyltransferase

Recombinant tRNA (guanine (37) -N1) -methyltransferase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP520650FHIYeast1mgQuote
EP520650FHIE. coli1mgQuote
BP520650FHIBaculovirus200ugQuote
MP520650FHIMammalian Cell200ugQuote

Protein Information

SpeciesPuccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)
UniProt IDE3KWE1
Gene NameTRM5; ORFs:PGTG_14821
Protein NametRNA (guanine (37) -N1) -methyltransferase
Region Expressed1-458
Expression Tag6xHis
Purity>90%
AA SequenceMLQTPQTGSLMLSSLLTTRRTISSMAALQQPSRHRFPPPNNIQRLPLRPDGQQEPIKLTE AIKQQFKQSIPLLAARVPSPSITKFKTNPEIRKALLNSKNLRSVEPDPDSTDNAFKLLLL NTPHKDQIPESTLKVFQEHDIQIKNTKLDLDWEYWMADEIIERLLPDQLTDIPASFTMIG HIAHFNLRDEYLPYKYLIGQVILEFRFFEMELLAGEPDYTVTLWQSGCRYRFDFSKVYYN PRLSTEHDLLSSMIEKDEVVVDAFAGVGPFAMRAAANRKAWVLASDLNPASVEALETNVR LNKLQGRVAVSGGDGREKIREAVRTLWLDKPFKTPNSSPLLPDHFIINLPDSSIQFLDAF RDLYHPLSDSEGFLNAVKKKSRLPLLHCYCFTKQVDEPESDICQRVSEVMKVEISPSTVA RFELKFVRAVAPHKDMYRITFELPLKLLFSSSSEESRA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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