$148.20Catalog Number: PTN328-20ugSize: 20ug

TRIM21 Human

Tripartite Motif Containing 21 (RO52) Human Recombinant

TRIM21 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 54,922 Dalton. TRIM21 is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.

Accession

P19474

Applications

Western blot with:
a) SLE sera (secondary Sjogren¡¯s syndrome).
b) Monoclonal anti-hexa-His-tag antibody.

Coating concentration

0.3-0.65 g/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.

Formulation

TRIM21 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.

Immunological Fuctions

1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Physical Appearance

Sterile Filtered clear solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Source

Sf9 insect cells.

Storage Conditions

Store at 4oC if entire vial will be used within 2-4 weeks. Store, frozen at -20oC for longer periods of time.
Avoid multiple freeze-thaw cycles.

Synonyms

52 kDa Ro protein, Sjoegren syndrome type A antigen, SS-A, Ro(SS-A), 52 kDa ribonucleoprotein autoantigen Ro/SS-A, Tripartite motif-containing protein 21, RING finger protein 81, TRIM21, RNF81, RO52, SSA1, SSA, RO-52.

Quantity

$148.20

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