EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP.
The TNFRSF12A biological activity is determined by its ability to inhibit TWEAK-induced weak cell death of HT29 cells. The expected ED50 for this effect is 1.0-5.0ug/ml in the presence of 1ug/ml rhTWEAK.
Lyophilized from a 0.2um filtered concentrated solution in PBS, pH7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 97.0% as determined by
(a) Analysis by HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized TNFRSF12A in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized TNFRSF12A although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution TNFRSF12A should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Tumor necrosis factor receptor superfamily member 12A, FN14, CD266 antigen, TweakR, tweak-receptor, Fibroblast growth factor-inducible immediate-early response protein 14, FGF-inducible 14, type I transmembrane protein Fn14.