Stimulates the growth of NRK-1 cells in soft agar at concentrations ranging from 0.1 to 5ng/ml corresponding to a specific activity of 0.2-10MIU/mg. Effective concentration ranges must be experimentally determined. Purified EGF and/or TGF-alpha at concentrations of at least 2ng/ml must be present for observation of the biological activity.
TGF-Beta1 protein was lyophilized from a solution containing 5mM Sodium Citrate (200ul) pH 3.5.
Sterile Filtered lyophilized powder.
It is recommended to reconstitute lyophilized TGF-beta 1 in sterile 100ul 5mM HCl, which can then be further diluted to the desired aliquot to 1ml of PBS containing 2mg/ml Albumin.
Lyophilized TGF-beta 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution TGF-beta 1 should be stored at 4oC between 2-7 days and for future use below -18oC.
DO NOT RECONSTITE WITH NEUTRAL BUFFERS.
DO NOT USE GLASS IMPLEMENTS OR EXTENSIVE MANIPULATIONS.
PREVENT FREEZE THAW CYCLES.
Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1.
