The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Asp-Thr-Asn.
The biological activity was determined using cell toxicity assay via HT-2 cells. The ED50 was found to be < 0.05ng/ml, corresponding to a specific activity of 20,000,000IU/mg.
The protein solution contains 20% Ethanol and 0.1% Acetic acid.
Sterile Filtered clear solution.
Greater than 97.0% as determined by SDS-PAGE.
Chinese Hamster Ovarian Cells.
TGF-beta 3 although stable at 4oC. for 3 weeks, should be stored at -20oC to -70oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.
