The sequence of the first five N-terminal amino acids of I-TAC was determined and found to be, Phe-Pro-Met-Phe-Lys.
ED50 range=1-10ng/ml corresponding to a Specific Activity of 10,000-100,000IU/mg determined by the dose dependent chemotaxis of human lymphocytes cultured in the presence of IL-2.
The I-TAC protein was lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized I-TAC in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized I-TAC although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution I-TAC should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Small inducible cytokine B11, CXCL11, Interferon-inducible T-cell alpha chemoattractant, I-TAC, Interferon-gamma-inducible protein 9, IP-9, H174, Beta-R1, chemokine (C-X-C motif) ligand 11, IP9, b-R1, SCYB11, SCYB9B, MGC102770.
