Request QuoteCatalog Number: xP510224GFJSize: 0.2-1mg

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Recombinant 2-succinylbenzoate--CoA ligase (menE)

Recombinant 2-succinylbenzoate--CoA ligase (menE) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP510224GFJYeast1mgQuote
EP510224GFJE. coli1mgQuote
BP510224GFJBaculovirus200ugQuote
MP510224GFJMammalian Cell200ugQuote

Protein Information

SpeciesGeobacillus sp. (strain WCH70)
UniProt IDC5D6U5
Gene NamemenE; Locus:GWCH70_2796
Protein Name2-succinylbenzoate--CoA ligase
Region Expressed1-492
Expression Tag6xHis
Purity>90%
AA SequenceMQTSIPNWLMQRAFLTPERIAVYDGQGKKTFMELHESVMKRARQLANTGVRKGDIVAIFM KNSVAMIECIHALHYIGAIVLLQNTRLTSHELAWQLKDSGAVYVIADDELADRIEGNIRV ITMSELSALPEENVEFQQYYHFDDIATIMYTSGTTGKPKGVLQTYENHWWSAIGSALNLG LNENDCWLAAVPFFHISGLSIMMRSVIYGMSMYVMRAFDAKKANELIIGGKVTIMSVVSA MLQKMIADLGERRYPETFRCMLLGGGPAPKPLLEVCKAKGIPVYQTYGMTETASQIATLA PEYSLTKLGSAGKPLFPSQLRIEKDGQVARPYEPGEIVVKGPNVTKGYLHRPDATAKAIR GGWFYTGDIGYIDEDGFLYVLDRRSDLIISGGENVYPAEIEAVLLSHEAVEEAGVTGIDD ETWGQVPCAFVKRKRGYSVTVEQLKQFCQAHLAKYKIPKQIYFVDELPRNASQKLLRHQL KQLIPNNENGAL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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