Streptavidin Recombinant, His Tag
Streptavidin Streptomyces Avidinii Recombinant fused to N-terminal His-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids and having a molecular mass of 17 kDa.
Accession
Amino Acid Sequence
MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ.
Formulation
The Streptavidin protein solution contains 20mM Tris-HCl pH7.5.
Physical Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Source
Stability
Streptavidin although stable 4oC for 4 weeks, should be stored desiccated below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.