Small Nuclear Ribonucleoprotein Polypeptide D3 Human Recombinant, Sf9
SNRPD3 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 14,739 Dalton. SNRPD3 is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Accession
Applications
Western blot with anti-Sm positive patient sera.
Coating concentration
0.4-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Formulation
SNRPD3 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.
Immunological Function
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Physical Appearance
Sterile Filtered clear solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Source
Stability
Store at 4oC if entire vial will be used within 2-4 weeks. Store, frozen at -20oC for longer periods of time. Avoid multiple freeze-thaw cycles.
Synonyms
Small nuclear ribonucleoprotein D3 polypeptide 18kDa, Sm-D3, snRNP core protein D3.
