Western blot with anti-Sm positive patient sera.
0.4-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.
SNRPD is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels).
Sterile Filtered clear solution.
Greater than 60% as determined by SDS-PAGE.
Store at 4oC if entire vial will be used within 2-4 weeks. Store, frozen at -20oC for longer periods of time.
Avoid multiple freeze-thaw cycles.