$75.00Catalog Number: PTN445-5ugSize: 5ug

SNRNP70 Human

Small Nuclear Ribonucleoprotein 70kDa Human Recombinant

Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein (lacking 66 internal amino acids outside the known epitope-containing areas) fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa (U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight and the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis).

Accession

P08621

Applications

Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera (Mixed Connective Tissue Disease).

Coating concentration

0.3-0.6 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.

Formulation

U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA and 0.02% SDS.

Immunological Function

1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Physical Appearance

Sterile Filtered clear solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Source

Escherichia Coli.

Storage

Store at 4oC if entire vial will be used within 2-4 weeks.
Store, frozen at -20oC for longer periods of time.
Avoid multiple freeze-thaw cycles.

Synonyms

U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP.

Quantity

$75.00

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