MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG.
Determined by it¡¯s ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts, with an expected ED50 in the range of 50-250ng/ml corresponding to a specific activity of 4,000-40,000IU/mg.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Sonic HedgeHog in sterile 18MΩ-cm H2O at a concentration of 0.1mg-0.5mg per 1ml.
Lyophilized Mouse Sonic HedgeHog although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Sonic HedgeHog should be stored at 4oC between 2-7 days and for future use below -18oC.
Please prevent freeze-thaw cycles.
SHH, HHG-1, HHG1, Sonic hedgehog protein.
