IIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG.
The ED50, determined by the dose-dependent induction of alkaline phosphatase production by C3H10T1/2 fibroblasts is 760ng/ml, corresponding to a specific activity of 1315.8IU/mg.
Lyophilized from a concentrated (1mg/ml) solution in water containing 10mM sodium Phosphate buffer pH-7.5.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Sonic HedgeHog with sterile water at a concentration of 0.1 - 0.5 mg/ml, which can be further diluted into other aqueous solutions.
Lyophilized Human Sonic HedgeHog although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Sonic HedgeHog should be stored at 4oC between 2-7 days and for future use below -18oC.
Please prevent freeze-thaw cycles.
SHH, HHG-1, HHG1, Sonic hedgehog protein, TPT, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5.
